Which protocol is NOT part of instrument optimization in flow cytometry?

In the context of flow cytometry, instrument optimization is focused on ensuring that the instrumentation is set up correctly to provide the best possible data quality. This often involves several protocols aimed at fine-tuning the performance of the instrument in terms of signal detection, efficiency, and accuracy.

Determining photoelectron efficiency, evaluating signal synchronization, and deducing laser delay determination are all directly related to optimizing how the flow cytometer detects and processes fluorescence signals from samples. Photoelectron efficiency refers to the ability of the detector to convert incident photons into electrical signals effectively, which is crucial for accurate measurements. Evaluating signal synchronization ensures that the timing of signal detection is synchronized with the flow of particles in the liquid stream, thereby facilitating accurate data collection. Deducing laser delay determination focuses on aligning the laser detection timing with the arrival of particles at the detector to ensure that signals are captured correctly.

Analyzing sample cytotoxicity, on the other hand, involves assessing the biological effects of a sample (e.g., how it affects cell health or function), which is more relevant to the biological interpretation of results rather than the instrument optimization process itself. While important for understanding the overall experiment, this assessment does not contribute to fine-tuning the performance of the flow cytometer. Hence