ASCP Specialist in Cytometry (SCYM) Practice Exam

When handling samples in closed tubes, it is important to use a biological safety cabinet if available. This is because biological safety cabinets provide a controlled environment that protects the user from potential exposure to biohazardous materials present in the samples. They are designed to filter and contain aerosolized particles that could be generated while vortexing, thereby minimizing the risk of contamination and ensuring both the safety of the operator and the integrity of the samples.

Using a biological safety cabinet enables the safe manipulation of potentially infectious or hazardous materials. It is essential in maintaining aseptic conditions and protecting the operator and the surrounding environment during procedures that could create aerosols. Vortexing in an open laboratory area can increase the risk of exposure to airborne particles, while vortexing closed tubes without appropriate containment does not provide the necessary safety measures.

Wearing gloves is important for personal protection but does not mitigate the risk of aerosol generation. Lastly, while there might be situations where vortexing is unnecessary, the correct handling of samples should facilitate thorough mixing when needed, and this should be done with appropriate safety precautions in mind. Thus, utilizing a biological safety cabinet is the best practice in this scenario.