What is the recommended density of media to isolate mononuclear cells from blood samples?

Isolating mononuclear cells from blood samples typically involves a technique called density gradient centrifugation, where the blood sample is layered over a density gradient media. The recommended density for such media is around 1.077 g/mL, which is optimal for the separation of lymphocytes and monocytes from plasma and red blood cells.

This specific density allows for effective stratification during centrifugation, enabling the mononuclear cells to form a distinct layer. The cells are then easily collected from the interface where the density of the separation media and the cells match. Using a density of 1.077 g/mL ensures minimal cell loss, preserves cell viability, and maintains the functional integrity of the isolated mononuclear cells, making it the standard choice in laboratory protocols.

Other densities, such as those mentioned in the other options, may not provide the ideal separation quality required for various downstream applications in cytometry or immunology.