What is the optimal storage medium for bone marrow biopsy or fine needle aspirate to maintain cell viability?

The optimal storage medium for bone marrow biopsy or fine needle aspirate to maintain cell viability is RPMI tissue culture medium. RPMI 1640 is a widely used cell culture medium that provides a balanced nutrient composition, including amino acids, vitamins, glucose, and salts necessary to support the growth and viability of hematopoietic cells. Its formulation is specifically designed to maintain the delicate balance of nutrients and pH, which are crucial for preserving cell integrity and functionality after the sample is collected.

In the context of preserving cell viability, RPMI tissue culture medium is preferable because it can provide the essential components for cells to survive and proliferate outside their native environment. It also contains a buffering system that helps maintain the optimal pH level, further contributing to cell viability.

Other options, while they may be suitable for specific circumstances, do not provide the comprehensive nutrient profile necessary for long-term storage of bone marrow cells. For instance, sterile saline is primarily used for rinsing or diluting specimens and lacks the essential nutrients that support cellular metabolism. Complete blood count solution is tailored for counting and may not be suitable for preserving cellular viability over time. Phosphate-buffered saline serves more to maintain pH and isotonic conditions but does not provide the nutritional support required