What contributes to the formation of a voltage pulse in detection?

The formation of a voltage pulse in detection is primarily influenced by the number of photons that are converted to electrons. In a detection system using photodetectors or photomultiplier tubes, when light (photons) strikes the detector, it results in the ejection of electrons, generating a measurable electrical signal. The more photons that are detected, the greater the number of electrons produced, leading to a larger voltage pulse. This relationship is fundamental to how detectors in cytometry quantify the presence and intensity of signaling substances or fluorescent markers. Essentially, the strength of the voltage pulse is a direct reflection of the amount of light received and, consequently, the number of photons converted to electrons.

Other factors, while they might influence the detection process, do not directly contribute to the formation of a voltage pulse in the same manner as photon-to-electron conversion does. For example, the size of the light source may affect the intensity and spatial profile of the light but does not determine the electrical response itself. Similarly, although the type of cell analyzed can influence the intrinsic properties of the fluorescence being measured, it does not affect the basic mechanism of voltage pulse formation. Thermal conductivity could relate to the efficiency and stability of the detection system but has no direct role in the