In a multiplex assay, what method is used to measure multiple analytes simultaneously?

The method that effectively measures multiple analytes simultaneously in a multiplex assay is based on the use of immunoassay techniques that incorporate magnetic beads. This approach utilizes the unique properties of magnetic beads that can be coated with specific antibodies targeting different analytes. The versatility of magnetic beads allows for the effective isolation and detection of multiple targets at the same time.

In this method, each type of bead can be functionalized with antibodies specific to different analytes. When a sample is introduced, various analytes present in the sample will bind to their corresponding beads. Following binding, the beads can be washed and then analyzed, typically using a flow cytometer, which allows for the detection of the different beads and thus the different analytes in a single sample run. This multiplexing capability is highly efficient and minimizes sample volume requirement while increasing throughput.

Other options involve different methods that are suitable for measuring analytes, but they do not provide the same level of multiplexing or are limited to single analyte detection per assay setup, making them less suitable for simultaneous analysis in a multiplex context. Magnetic bead-based assays are specifically designed for this purpose, making this approach particularly powerful in research and clinical applications where analyzing numerous biomolecules is required.